Virus que infectan naturalmente bacterias y se comportan como parásitos intracelulares obligados.
Así se les denomina a las secuencias variables en el DNA que hacen que cada individuo sea único.
Es el primer paso dentro de los ciclos de una PCR.
Molécula que hace posible la identificación de individuos de acuerdo a su parentesco por línea materna.
Agente utilizado en la extracción de ácidos nucleicos, solubiliza las membranas celular y nuclear provocando su lisis.
Polimorfismo que contiene la variación de un sólo nucleótido o par de bases en una secuencia dada.
Presentan actividad endonucleasa cortando los enlaces fosfodiéster del DNA en una secuencia diana específica.
Vectores cuya finalidad es el almacenamiento de secuencias y la obtención de grandes cantidades del ADN insertado.
Muestra requerida cuando se desea determinar el nivel de expresión génica.
Pequeñas moléculas circulares de ADN que se replican y se transmiten con independencia del cromosoma bacteriano.
Polisacárido extraido de algas marinas que es ampliamente utilizado en la electroforesis.
Es uno de los métodos para extracción de ácidos nucleicos.
Down
Método de cuantificación de ácidos nucleicos.
Vectores cuyo objetivo es producir un transcrito (ARN) o la proteína producto de ese transcrito.
Electroforesis empleada exclusivamente con geles de poliacrilamida.
Técnica que amplifica las secuencias de ADN en dos rondas de amplificación con distintos pares de iniciadores o primers en cada una.
Así se les conoce a los polimorfismos de longitud de fragmentos de restricción.
La detección de las copias del producto de PCR se realiza al mismo tiempo que sucede la amplificación.
Método de secuenciación que emplea análogos de dNTP específicos de terminación de cadena.
Constituyen una distribución ordenada de más de 10 mil genes por cm2 en una pequeña superficie en el cual pueden estudiarse simultáneamente mediante hibridación múltiples genes.
Consiste en la migración de moléculas a través de una matriz porosa por acción de un campo eléctrico.
Segmentos de ADN o ARN de cadena sencilla marcados con moléculas reporteras que permiten su fácil detección.
Es una estrategia estándar para analizar ADN previamente digerido con enzimas de restricción.
Muestra requerida para la identificación de una mutación o polimorfismo en la estructura genómica de un individuo.
Consiste en la amplificación in vitro del DNA.
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